DNA bands in gel were visualized under UV light. 1999).The PCR products were separated electrophoretically in 1.5% agarose gel and stained with Simply Safe (EURx, Polska). The recognition of species were provide by multiplex PCR method according to European Reference Laboratory method (Zarlenga et al. The DNA from each larvae were separately extracted using commercial kit according to manufacturer protocol (Promega, USA). nativa in wild boar in Poland (and Central Europe) we aimed to perform the genetic analysis of isolated larvae.įor the study, five larvae extracted from the muscle of the positive wild boar were used. Intensity of invasion was evaluated as one larvae per 10 g of muscle tissue. The infected wild boar was female, close to 2 years old, and weight of carcass after removing of the guts were about 40 kg. nativa was originated from wild boar gained in Ławice, district Iława, Varmian Masurian province. britovi (19%), and in 7 samples the mixed infection T. Altogether, 235 samples were identified as T. The species identification was done by multiplex PCR according to the protocol of European Reference Laboratory for Parasites. 2013).įrom January to September 2016, 300 muscle samples from wild boars previously recognized by VIS laboratories as Trichinella positive were delivered to NVRI for species identification. Until now, this species was detected in Poland only once in red fox (Chmurzynska et al. nativa discovered in few Northeastern European countries, was never detected in wild boars in Poland. pseudospiralis were also detected (Bilska-Zajac et al. britovi were discovered in wild boars in Poland (Bilska-Zając et al. With the increase of the population, the number of Trichinella-infected animals is rising and now achieved over 600 positive animals per year. It should be taken into consideration that the wild boar population in Poland increased from the last two decades from 90,000 up to 300,000 (Popczyk 2016). It should be stressed that in the last decade, human trichinellosis was caused by ingestion of meat from illegally hunted and not-examined Trichinella-infected wild boars only. From four routinely examined animal species, trichinellosis is discovered the most often in wild boars. The National Veterinary Research Institute as a national reference laboratory for trichinellosis in Poland is responsible for confirmation of positive results and species identification. In Poland routine inspection (detection of parasites) of meat from pigs, wild boars, coypus, and horses is provided by veterinary inspection services. Therefore, compulsory examination for trichinellosis in accordance with Commission Implementing Regulation (EU) 2015/1375 of 10 August 2015 laying down specific rules on official controls for Trichinella in meat, has two phases and includes meat inspection to detect larvae and, in the case of positive results, identification of Trichinella species causing the invasion. Information on Trichinella species occurring in the country is also important for veterinary services of third countries in the supervision of imports of food of animal origin. 2016) resulted in the limitation of the compressor method use in endemic regions. pseudospiralis in wild boars in Poland (Bilska-Zajac et al. These characters influence the risk assessment in humans and may affect the development of new sanitary and veterinary regulations. Individual species differ by their properties and zoonotic potential. All of them are infective to human and consumption of meat with live larvae poses a risk of trichinellosis for humans. From these 12 known taxa, 4 are circulating in Europe, 3 encapsulated ( Trichinella spiralis, Trichinella nativa, Trichinella britovi) and one non-encapsulated ( Trichinella pseudospiralis) (Pozio and Zarlenga 2013). Till now, 12 taxa in this genus are recognized and divided into two clades: encapsulated and non-encapsulated ones. L200 (KB_T, KA_T) (2005>) 2.4I L 200 (KB _ T, KA _ T) P 2400 4G64 L200 II (KB4T/KA4T)) (2005>) 2.5 DI-D 2WD, 2.5 TD 4WD, 2.5 TD 4WD Hi- Power D 2477 4D56 L200 SPORTERO (2008>) L200 Sportero 2.Nematodes of the genus Trichinella are zoonotic parasites with a high impact on human health and animal trade (Gottstein et al. PAJERO SPORT II (KH _, KG _ ) 3.2 DI-D 4WD PAJERO DAKAR II (2008>) PAJERO DAKAR II (KH _, KG _ ) 3.2 DI-D 4WD NATIVA II (2008>) NATIVA II (KH _, KG _ ) 3.2 DI-D 4WD MONTERO SPORT II (2008>) MONTERO SPORT II (KH _, KG _ ) 3.2 DI-D 4WD D 3200 4M41 CHALLENGER II (KH _, KG _, PB, PC) 3.2 DI-D 4WD
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